I should explain technique of what may be called field stacking, i.e photographing at multiple planes of focus, off-hand. Fly's will only give you one or two seconds as you approach, so a longer lens, here about 600mm., by using supplementary optics, (equals longer working distance,) is an essential benefit. Also magnification can thus be pushed beyond 1:1. Secondly focusing is fixed and and the camera is moved, (best to move the entire body,) as you trip the shutter. In the brief time the subject freezes for a second or so, it is possible to get half a dozen shots off, continuously, starting at the front of the subject and moving back (or vice versa.) Sometimes very small movement artifacts can be corrected when the multiple exposures are combined in photo stacking software. For this image, I only fired off two shots because I still wanted fairly shallow focus depth. Just another shot for extra measure! :)